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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in many tumors, which ends up in chromosome instability, and therefore may lead to tumorigenesis. This protein was observed to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a critical part in endorsing cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been explained. Two more splice variants have been documented but thorough nucleotide sequence data just isn't however accessible.
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Blocking peptides are peptides that bind precisely towards the goal antibody and block antibody binding. These peptide typically contains the epitope identified via the antibody.
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This guidebook outlines additional doable optimization of mobile and immunoassay parameters to ensure the very best success are...
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This information outlines more doable optimization of cellular and immunoassay parameters to guarantee the very best benefits are...
Antibodies sure to the blocking peptide not bind to the epitope about the target protein. By comparing the staining within the blocked antibody compared to the antibody by itself, one can see which staining is distinct.
Target Details Cyclin E belongs into the extremely conserved cyclin spouse and children, whose members are characterized by a spectacular periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation styles which lead to your temporal coordination of every mitotic celebration. Cyclin E sorts a complex with and features as a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S period boundary and is also degraded as cells progress by means of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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